This study was aimed to analyze the antigenicity of T. vaginalis using a high performance gel filtration chromatography(HPLC), enzyme-linked immuno-sorbent assay(ELISA) and enzyme-linked immunoelectroctransfer blot(EITB). Soluble antigen was
prepared
uring a bead beater. Antiserum was collected from rabbit immunized previously. The results obtained are as follows ; the crude antigen of T. vaginalis was separated into 7 peaks by HPLC and the fraction 3 revealed the highest reactivity in ELISA.
The
immunogenic bands of fraction 3 were visualized at 38, 45, 47, 55, 78, 94, 115, 126 and 180 KDa and the bands at 38 KDa and 180kDa showed stronger reactivity than that of equivalents of the crude antigen in EITB.
In conclusion the fraction 3 of the crude antigen of T. vaginalis obtained by chromatographic sepaaraction may have high antigenicity and proteins by 28KDa and 180KDa of the fraction 3 may play an important role in antigen-antibody reaction in T.
vaginalis infection.
|